All You Need to Know About an HPLC and How it Works

An HPLC, or High Performance Liquid Chromatography, is a chromatographic process that is used to separate a mixture in analytical and biochemistry needs, to classify, measure, and/or clarify the individual components within the mixture.
Solvent/Mobile Phase Reservoir
A reservoir holds the components or solvent, which then moves to a high-pressure pump.
Pump Solvent Manager Solvent Delivery System (Mobile Phase)
The pump generates and meters the specified flow rate for the solvent, usually measured in milliliters per minute.
Sample/Injector AutoSampler Sample Manager
An injector is then used to retrieve and add the sample into the solvent stream that transports the sample into the HPLC column.
HPLC Column (Stationary Phase)
The chromatographic packing material, also known as the stationary phase, within the column then assists with the separation of the materials.
Detector
A detector is used to collect data and see the separated compound bands as they elute, or are removed, from the HPLC column.
Waste
The solvent then exits the detector and is either sent to waste or collected through a fraction collector if needed.
Fraction Collectors
The fraction collector gathers the sample components for further analysis after the HPLC process. In this process, the liquids are moved from one vial to another and can be set up for timed events.
Degasser
The degasser removes gas throughout the HPLC process.
Column Heater
The column heater heats the HPLC column during the HPLC process.
Interface Cards
Interface cards are used to communicate between a computer and the HPLC equipment
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